microbeam - x-ray microtomography, targeted irradiation, cells irradiation
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Welcome to the x-ray microbeam site!
Here you can learn about microbeams and our facility construction. At our microprobe two experiments are performed: x-ray microtomography and targeted irradiations. Both experimental lines use one common x-ray source, which is an open type x-ray tube with microfocusing. Radiation is emitted from the spot, which has the size of about 2 micrometers in diameter. This give us high resolution in microtomography (10 micrometers) and enable precise targeted irradiation after refocus the cone beam outcoming from the source.


Microfocus X-ray computed tomography (uCT) is a non-destructive method widely applied in various disciplines. It reveals the internal structure of investigated objects, determined by variations in density and atomic composition.

x-ray microtomography basic concept

Microfocus X-ray computed tomography has recently emerged as an important and powerful tool because it is relatively easy to apply providing at the same time fine spatial resolution (about 10 um).

Visit the Microtomography gallery

microtomographic image of transistor
Image of transistor sample. Small connections between base, collector and emmiter are well visible.

Click here to see the full 3D reconstruction of the object.

Microdosimetry and targeted irradiation

In order to irradiate microscopic objects the radiation comming from the source is being refocused on the sample.

The diameter of focused beam is less than 20 um, and the minimal repeateable step of sample positioners is less than 1 um. Fast beam shutter set a precise time of irradiation (min. 120 ms).

Visit the Cells irradiation gallery

X-ray litography. Letters IFJ irradiated on stomatologic x-ray film. The beam is wider due to distraction on the film cover.

The first application is microdosimetry - analysing the behavior of biological cells after irradiation. The sample is observed with the use of optical microscope (resolution 3 um), equipped with camera.

Image of cells

Faculty of Applied Spectroscopy, Institute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, Kraków, Poland